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科研細胞
藥靶細胞
標準品
41BB/NFkB-Luc/HEK293
| CBP74045 | |
| I. Background | |
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Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients. |
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| II. Description | |
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Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry. |
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: |
4-1BB |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | 4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): |
Screen for activators or inhibitors of CD137 signaling in a cellular context and characterize the biological activity of CD137 and its interactions with ligands |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB. |
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Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold. |
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