Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.
Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14|
|Freeze Medium:||90% FBS+10% DMSO|
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Screen for activators or inhibitors of CD137 signaling in a cellular context and characterize the biological activity of CD137 and its interactions with ligands
|IV. Description of Host Cell Line|
|Ⅴ. Representative Data|
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB.
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold.
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